Abstract
BackgroundOur previous study demonstrated that the composition of the urinary microbiota in female patients with type 2 diabetes mellitus (T2DM) was correlated with the concentration of urinary interleukin (IL)-8. As the composition of urine is mainly determined by diet, diet might mediate the correlation.MethodsSeventy female T2DM patients and 70 healthy controls (HCs) were recruited. Midstream urine was used for the urine specimens. Urinary IL-8 was determined by enzyme-linked immunosorbent assay. A Chinese Food Frequency Questionnaire was used to collect food intake data. The independent variables in the hierarchical regression analysis were the relative abundances of the bacterial genera and species that were significantly different between the T2DM and HCs and between the T2DM patients with and without detectable urinary IL-8, and the bacterial genera associated with IL-8 concentration in the multiple regression model reported in our previous research. IL-8 concentration was the dependent variable, and nutrient intakes were moderator variables.ResultsFiber and vitamin B3 and E intake exerted enhancing effects, and water intake exerted a buffering effect, on the positive relationship between the relative abundance of Ruminococcus and IL-8 concentration (p < 0.05). Cholesterol and magnesium intake exerted enhancing effects on the positive relationship between the relative abundance of Comamonas and IL-8 concentration (p < 0.05).ConclusionModulating T2DM patients’ dietary patterns may prevent bladder inflammation.
Highlights
Diabetes mellitus (DM) is a severe chronic disease that is, recognized as a global public health problem
Recruitment of subjects Briefly, a case-control design was used in our previous study, in which female type 2 diabetes mellitus (T2DM) patients were individually matched to healthy controls (HCs) according to age, marital status, and menstrual status
The strongest positive association between the relative abundance of Ruminococcus and IL-8 concentration occurred in patients who reported a high vitamin E intake, the weakest association occurred in patients who reported a medium vitamin E intake, and a negative association was apparent in patients who reported a low vitamin E intake (Fig. 1D)
Summary
Diabetes mellitus (DM) is a severe chronic disease that is, recognized as a global public health problem. Studies on the urinary microbiota have caused this concept to be questioned over the past decade, as DNA sequencing and expanded quantitative urine culture technique have shown that bacteria live in almost all individuals’ bladders, both UTI patients and healthy subjects (Price et al, 2016; Thomas-White et al, 2018). Our previous research demonstrated that T2DM patients had lower bacterial diversity in urinary microbiome than healthy controls (HCs). There were significant differences in the relative abundance of multiple urinary bacterial genera between T2DM patients and HCs, including Lactobacillus, Prevotella and Pseudomonas (Liu et al, 2017). The urinary microbiome profile in T2DM females is different from HCs, and the bacterial dysbiois is related to the expression level of IL-8. To date, no studies have shown whether the relationship between urinary microbiome and IL-8 level is regulated by nutrients intake. If nutrients intake has a moderating role, clinicians can precisely adjust patient’s dietary composition based on the correlation between urinary microbiome and IL-8 concentration
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