Moderate Photoinhibition of Photosystem II Significantly Affects Linear Electron Flow in the Shade-Demanding Plant Panax notoginseng.

Wei Huang,Tao Liu,Shi-Bao Zhang

doi:10.3389/fpls.2018.00637

Abstract

Although photoinhibition of photosystem II (PSII) frequently occurs under natural growing conditions, knowledge about the effect of moderate photoinhibition on linear electron flow (LEF) remains controversial. Furthermore, mechanisms underlying the decrease in LEF upon PSII photoinhibition are not well clarified. We examined how selective PSII photoinhibition influenced LEF in the attached leaves of shade-demanding plant Panax notoginseng. After leaves were exposed to a high level of light (2258 μmol photons m-2 s-1) for 30 and 60 min, the maximum quantum yield of PSII (Fv/Fm) decreased by 17 and 23%, respectively, whereas the maximum photo-oxidizable P700 (Pm) remained stable. Therefore, this species displayed selective PSII photodamage under strong illumination. After these treatments, LEF was significantly decreased under all light levels but acidification of the thylakoid lumen changed only slightly. Furthermore, the decrease in LEF under low light was positively correlated with the extent of PSII photoinhibition. Thus, the decline in LEF was not caused by the enhancement of lumenal acidification, but was induced by a decrease in PSII activity. These results indicate that residual PSII activity is an important determinant of LEF in this shade-adapted species, and they provide new insight into how strong illumination affects the growth of shade-demanding plants.

Highlights

During oxygenic photosynthesis in plants, leaves absorb light energy to drive photosynthetic electron flow in the chloroplasts
We focused on the mechanism underlying the decrease in linear electron flow (LEF), and we investigated the relationship between low-light LEF and photosystem II (PSII) activity
These results demonstrated that this shade-adapted species P. notoginseng shows selective PSII photoinhibition under high-light stress

Summary

Introduction

During oxygenic photosynthesis in plants, leaves absorb light energy to drive photosynthetic electron flow in the chloroplasts. Photosystem II (PSII) is very sensitive to high-light stress because D1 protein is decreased in the PSII reaction centers and OEC activity is subjected to PSII Photoinhibition and Linear Electron Flow photodamage (Aro et al, 1993a,b; Hakala et al, 2005; Ohnishi et al, 2005; Takahashi et al, 2009). This phenomenon, photoinhibition of PSII, can influence photosynthetic electron flow and overall plant growth (Kulheim et al, 2002; Murchie and Niyogi, 2011; Tikkanen et al, 2014)

Results

Discussion

Conclusion