Models of Ischaemia and Ischaemia– Reperfusion of the Myocardium in an Experiment on Rats with Regard to of Novel Concepts of Progression of Acute Coronary Syndrome

Abstract

Purpose: Animal models of cardiovascular diseases provide us with important insights regarding the pathophysiology and progression of cardiovascular diseases and are instrumental in evaluating new pharmacological agents. To date, different models that focus on various aspects of acute coronary syndrome have been proposed. In this context, the primary aim of the present study was to investigate the models of total myocardial ischaemia and ischaemia–reperfusion from the standpoint of modern concepts of the development and progression of acute coronary syndrome. Patients and methods: The in vivo study involved 70 sexually mature male Wistar rats. Laboratory animals were divided into two groups of total myocardial ischaemia (n = 30) and myocardial ischaemia–reperfusion (n = 30). A group of intact rats (n = 10) was used to confirm that all lesions were caused directly by irreversible ischaemia or ischaemic–reperfusion syndrome (IRS) due to ligation of the left coronary artery.ResultsThe main histological, echocardiographic, biochemical changes in the myocardium in models of acute coronary syndrome are described. The main marker indicators are highlighted. Conclusion:The available range of experimental techniques allows the adaptation of the universally acknowledged model of acute coronary syndrome for the study of IRS, which in turn can be used to study the mechanisms of this pathological process and for preclinical studies of new treatment modalities influencing the cardiovascular system.