Modelling the In Vitro Growth of Phytopathogenic Filamentous Fungi and Oomycetes: The Gompertz Parameters as Robust Indicators of Propolis Antifungal Action.

Abstract

Propolis is a resinous mixture produced by honeybees, mainly from plant exudates. With a rich chemical composition including many phenolic compounds, mostly responsible for its biological properties, namely antimicrobial ones, propolis may be a promising alternative to synthetic pesticides. The study of propolis from the south of Portugal and of its potential against phytopathogenic agents are still very recent and different methodological approaches hinder a comparison of efficacies. In this context, we aimed to test the value of a mathematical model for the multiparametric characterization of propolis' antifungal action on solid medium assays. An ethanol extract (EE) of a propolis sample harvested in 2016 from Alves (A16) was characterized in terms of phenolic composition and antimicrobial potential against five phytopathogenic species. A16.EE (500-2000 µg/mL) inhibited the mycelial growth of all the species, with Phytophthora cinnamomi and Biscogniauxia mediterranea being the most susceptible and Colletotrichum acutatum being the least affected. The Gompertz mathematical model proved to be a suitable tool for quantitatively describing the growth profiles of fungi and oomycetes, and its parameters exhibit a high level of discrimination. Our results reveal that propolis extracts may have potential applications beyond traditional uses, particularly within the agri-food sector, allowing beekeepers to make their businesses more profitable and diversified.