Abstract
Experimental verification of the internal pore and external film diffusion model is presented using the oxidation of propene to propene oxide by calcium alginate-entrapped Mycobacterium cells as a model system. Assuming pseudo-one-substrate Michaelis-Menten kinetics and negligible product inhibition, theoretical consumption rates for oxygen and propene the immobilized cells were compared to experimental values obtained in a circulation batch reactor system. The diameter of the spherical gel particles, immobilized cell density and the intrinsic kinetic parameters were measured independently. Internal pore diffusion of the limiting substrate was found the main mass-transfer resistance. A good agreement was observed between predicted and experimental epoxidation rates.
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