Abstract
The decidualization of stromal cells is a key step in acquiring endometrial receptivity to the embryo. This process is regulated by sex steroid hormones, and in the mouse is additionally induced by embryo attachment. There are many protocols for the induction decidualization of stroma in vitro, but most of them require verification of the compliance of the processes occurring in cell culture with those in a living organism. This work focuses on the morphology of stromal cells and the expression activity of genes responsible for the progression of decidualization in 2D culture with subsequent embryo attachment. The feasibility of using several variants of protocols for the induction of decidualization and the possibility of using stromal cells after cryopreservation were also investigated.
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