Abstract
, it is possible to model and elucidate the interaction among the diverse metabolicpathways and their acetylation patterns.Mitochondrially enriched fractions from HCT116 colon cancer cells treated with 10mM propionate and/or butyrate in a 2 · 2 factorialdesign with two independent repeats were analysed using a label-free worksow. The total proteome and acetylated proteome wereidentiÞed and the data interrogated qualitatively as a Þrst step using ProteinLynxGlobal Server and Scaffold software packages. Repre-sentation analysis was undertaken DAVID and Reactome. Enzymes for major metabolic pathways involving acetyl-CoA (as a component:Glycolysis, Tricarboxylic Acid, Pyruvate metabolism and b -oxidation) were searched for by Uniprot identiÞer.Representation analysis indicated translation pathways were particularly enriched in the global proteome ( P = 1.6 · 10
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