Abstract
Tributyltin (TBT) is one of the most toxic substances ever deliberately introduced into the marine environment. The high toxicity of TBT has resulted in a wide range of adverse effects on biological systems ranging from bacteria to mammals and from the molecular to the community level. One of the most deleterious effects of TBT is imposex. The growth kinetics of TBT-Resistant Bacterium containing lead was studied. In this study various lead concentrations ranging from 1 to 100 mg/dm 3 were used. Seven kinetic models (Teissier, Monod, Yano, Luong, Aiba, Webb, and Haldane,) were investigated and the accuracy of the fitted models were evaluated using statistical analysis such as coefficient of determination, adjusted coefficient of determination (R 2 ) and root mean square (RMSE). Aiba model was fitted to the experimental growth kinetics data and gave a very good fit with an R 2 of 0.98 and RMSE of 0.0042 respectively. The calculated value for the Aiba constants such as maximal growth rate, half saturation constant and half inhibition constant rate symbolized by μmax, k s , and k i , were 0.038 hr -1 , 0.38s mg/dm 3 and 34.38 mg/dm 3 respectively. This is the first report of growth kinetics of TBT-Resistant bacterium by Klebsiella sp. FIRD 2 Containing lead. Keywords : Growth Kinetic models, Klebsiella sp. FIRD 2, lead, TBT-resistant bacteria.
Highlights
Due of its toxicity to marine organisms, the International Maritime Organization (IMO) in 2008 totally contraband the use of TBT as a constituent of antifouling paints
This paper describes the effect of various lead concentrations on the growth kinetics of TBT-resistant bacterium; Klebsiella sp
One of the valuable tool in biotechnology is the relationship between the specific growth rate (μ) of a population of microorganisms and the substrate concentration (S) which are represented by a set of empirically derived rate laws referred to as theoretical models
Summary
Bacterial strain: Previously, isolated TBT-resistant bacterium from contaminated surface sediment at Kong Kong Laut along Strait of Johor, Malaysia. In addition to the above compositions, the media contains 1000 μg/L of TBT and various concentrations of lead. Samples were drawn after every 6 hr and TBT growth containing lead was measured. The seed culture was transferred to 25mL of TBT liquid media containing various initial lead concentrations ranging from 1 to 100 mg/dm in the 250 mL Erlenmeyer flask. Resistant bacterium containing lead completely stops (mg/dm3), cell growth rate (hr-1), maximum cell growth rate (hr-1), saturation constant or half velocity constant (mg/dm3), inhibition constant (mg/dm3), and the exponent representing the impact of the substrate to μmax, respectively. For each initial concentration of lead, specific growth rate was calculated based on the linear portion of the growth against time in an exponential phase. ABDUSSAMAD, ABUBAKAR; ABDULLAHI, MUHAMMAD; DAYYABU SHEHU; MURTALA, YA’U; ABBA, BABANDI; ABUBAKAR, SADIQ TANKO; HADIZA, IBRAHIM; USMAN, SUNUSI; FERDAUS, MOHAMAT-
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