Abstract
BackgroundThe modelling framework is proposed to study protection properties of antibodies to neutralize the effects of the plant toxin (ricin). The present study extends our previous work by including (i) the model of intracellular transport of toxin to the Endoplasmic Reticulum and (ii) the model of the internalised antibodies (when antibody is delivered directly into the cytosol).MethodSimulation of the receptor-toxin-antibody interaction is implemented by solving the systems of PDEs (advection-diffusion models) or ODEs (rate models) for the underlying transport coupled with mass-action kinetics.ResultsAs the main application of the enhanced framework we present a comparative study of two kinds (external and internalised) of antibodies. This comparison is based on calculation of the non-dimensional protection factor using the same set of parameters (geometry, binding constants, initial concentrations of species, and total initial amount of the antibody).ConclusionThis research will provide a framework for consistent evaluation and comparison of different types of antibodies for toxicological applications.
Highlights
The modelling framework is proposed to study protection properties of antibodies to neutralize the effects of the plant toxin
In the present paper we extend our previous work [5,6,7,8,9] by refining models for intracellular transport and chemical interaction of species
We present a new modeling framework to evaluate the protective potential of various antibodies. This framework is based on the equation for the diffusion- and advectiondiffusion transport of all species in the extracellular and intracellular domains and include mass-action kinetics for toxin, receptor and antibody
Summary
The modelling framework is proposed to study protection properties of antibodies to neutralize the effects of the plant toxin (ricin). The following new processes have been included in our simulations: (i) the intracellular traffic that captures the diffusion of all species and the main features of the toxin microtubule transport, (ii) chemical interaction of all species inside the cell
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