Abstract
A relatively new field in biotechnology is the cultivation of animal cells in large scale bioreactors. For this, particular requirements for reactor design, process control and careful treatment of the cells must be met.In this paper, modelling and control strategy are presented for a 30 1 and a 70 l-bioreactor with a special bubble-free membrane aeration system. The plants are used for repeated batch and continuous cultivations of modified mouse L 929 Tk-cells.The cells are growing on micro-carriers and producing glycosilated human interferon-B constitutively. For a mathematical description of the plant, a lumped parameter model of the reactor liquid phase and a distributed parameter model of the bubble-free aeration system was developed. The latter includes mass balances of the gas phase and of gas exchange with the fermentation broth. The control strategy is based on system analysis with this model and the design steps of the control are explained. The control algorithm was implemented on a freely programmable DDC-process-station MICON P-200. (Manufacturer: VDO, Hannover, Germany.
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