Modelling aldehyde oxidase activity in aqueous-organic solvent mixtures at various temperatures

Abstract

A model is proposed to represent the enzyme activity ratios in water – organic solvent mixtures at various temperatures. The prediction capability of the model was evaluated employing aldehyde oxidase as a model enzyme in seven water – organic solvent mixtures at 25, 35 and 45°C by using mean percentage deviation (MPD). The MPD obtained for each water – organic solvent mixture was 6.2%. A general model was also proposed employing the Abraham solvent parameters with MPD as 19.5%. The proposed models could be used in industry for speeding up the process designs where water – organic solvent mixtures at various temperatures were employed.