Abstract
The response of a peroxidase-based optical biosensor was modelled digitally.A mathematical model of the optical biosensor is based on a system of non-linear reaction-diffusion equations. The modelling biosensor comprises two compartments, an enzyme layerand an outer diffusion layer. The digital simulation was carried out using finite differencetechnique. The influence of the substrate concentration as well as of the thickness of both theenzyme and diffusion layers on the biosensor response was investigated. Calculations showedcomplex kinetics of the biosensor response, especially at low concentrations of the peroxidaseand of the hydrogen peroxide.
Highlights
Biosensors are sensors made up of a combination of a biological entity, usually an enzyme, that recognizes a specific analyte and the transducer that translates the biorecognition event into a signal [1, 2]
Mathematical modelling in the design of optical biosensors has been applied in individual cases only [32, 33]
We consider the reaction scheme of the optical biosensor involving hydrogen peroxide (H2O2) reaction with peroxidase (E) to form compound I and water (H2O) with the constant reaction rate k1
Summary
Biosensors are sensors made up of a combination of a biological entity, usually an enzyme, that recognizes a specific analyte and the transducer that translates the biorecognition event into a signal [1, 2]. Optical biosensors are known to be suitable for environment, clinical and industrial purposes [7]. Those devices allow real-time analysis of molecular interactions without labelling requirements [8]. Mathematical modelling in the design of optical biosensors has been applied in individual cases only [32, 33]. The goal of this investigation is to make a model allowing an effective computer simulation of peroxidase-based optical biosensors as well as to investigate the influence of the physical and kinetic parameters on the biosensor response. By changing input parameters the output results were numerically analyzed at transition and steady state conditions
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