Abstract
Glucose 6-phosphate dehydrogenase is one of many enzymes found in Lactic Acid Bacteria (LAB). Leuconostoc mesenteroides strain K7 has been isolated from Kelengkeng (Dimocarpus longan) fruit. Kelengkeng fruit can generate LAB in previous research. The type of microbial isolate in form of the acidophilic microbe with pH 2.0 till 5.0 and it has macroscopic and microscopic characteristics is LAB and able to generate antimicrobial activity. The amplification results of the PCR technique showed glucose 6-phosphate dehydrogenase gene from L. mesenteroides strain K7 of 1112 pb as glucose-6-phosphate dehydrogenase (L. suionicum) with a similarity percentage of 99.41%. The modeling structure with SWISS-MODEL server was obtained of 486 amino acids with MSA analysis result of target protein sequence was LTGGGDKANDDAVEDDANNSATDAS FLADGTNSATMPSKA. It means that the sample variance is very significant and protein database to glucose-6-phosphate-1-dehydrogenase. The 3D modeling predictions of sequence identification results from target protein isolate and template (1h9a.1.A) have a similarity of 91.55% by glucose 6-phosphate dehydrogenase.
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