Abstract
The study of the role of viral-bacterial associations in the etiology of acute intestinal infections is considered new to medical microbiology. The purpose of the study was to determine the effect of viral-bacterial associations on the manifestation of virulence of pathogens and the degree of development of structural-morphological disorders of the internal organs in animals with antibiotic-induced dysbiosis. In a study of 210 white laboratory mice, BALB/c lines formed dysbiosis using antibacterial agents (ampicillin, gentamicin, metronidazole) followed by simulation of the experimental infection. To simulate salmonella infection, mice were infected with a clinical strain of Salmonella typhimurium intraperitoneally. Similarly, the animals were infected with Coxsackie B3 test culture virus (dose 106 TCD50). The sensitivity of mice to Coxsackie B and salmonella viruses was examined for mortality and disease characteristics. The animals were removed from the experiment 24 h after infection, electronically microscopically studied structural and morphological changes of the internal organs were performed. There was no statistically significant difference in morbidity (23.33-26.66 %) and mortality (16.66-20.0 %) of mice infected with Coxsackie virus with dysbiotic disorders and preserved microflora. Dysbiotic conditions have been shown to lead to associated viral-bacterial infections in animals and, accordingly, an increase in the incidence of disease and death in experimental animals. Against the background of disturbance of the composition of the normal intestine microbiota in viral bacterial infections, pronounced degenerative changes in the internal organs of animals were established, with signs of generalization. Electrogram data showed the appearance of activation of immunocompetent cells of the body in viral-bacterial infection in animals with impaired intestine microbiocenosis.
Highlights
Acute intestinal infections (AII) remain one of the world's most pressing health care issues [19]
Viruses are the cause of acute gastroenteritis due to noroviruses, which account for almost a fifth of AII; rotaviruses, especially in young children, cause more than 200,000 deaths annually worldwide [1, 6, 8]
In the group of animals in which virus-bacterial infections were modeled against the background of pronounced antibiotic-induced dysbiosis, there was an increase in the death of animals by 20.0 % and an increase in the incidence of disease by 16.66 %, compared with the group of experimental animals with viral-bacterial infections conducted the experiment without the formation of dysbiotic states
Summary
Acute intestinal infections (AII) remain one of the world's most pressing health care issues [19]. According to WHO, about 1.7 billion cases of acute diarrhea are reported annually alone in children [1]. Among microorganisms of bacterial nature, the main agents of AII are representatives of the family Enterobacteriaceae [14, 15]. Viruses are important etiologic factors of AII in children, especially in early childhood. Viruses are the cause of acute gastroenteritis due to noroviruses, which account for almost a fifth of AII; rotaviruses, especially in young children, cause more than 200,000 deaths annually worldwide [1, 6, 8]. Enteroviruses occupy a significant niche in this respect [9, 13]
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