Modeling of the absorption spectrum of Phaeodactylum tricornutum Bohlin in the red area

Abstract

Mathematical decomposition of the absorption spectrum of the culture of the red microalga Phaeodactylum tricornutum Bohlin was carried out in the spectral range from 550 to 750 nm. The culture spectra were recorded on a spectrophotometer with an integrating sphere. Using the literature data, mathematical models of the absorption spectra in the red area of the following pigments were compiled: chlorophyll a and chlorophyll с. The mathematical model of each pigment represented the sum of Gaussian curves. The resulting equations were taken as a basis for modeling the native spectrum. A general model of the absorption spectrum of culture in the region of 550–750 nm has been compiled, consisting of the sum of individual pigments. Using the model obtained in practice, it is possible to determine the concentrations of chlorophyll a and c directly from the absorption spectrum of the culture.