Modeling of protein adsorption to DEAE sepharose FF: Comparison of data with model simulation

Abstract

The equilibrium and kinetic characteristics of the adsorption of human serum albumin (HSA) and ovalbumin (OVA) to the DEAE Sepharose FF weak anion exchanger were experimentally determined. The rate for protein adsorption was simulated with two different models, the first being based on a single lumped kinetic parameter, while the second includes the individual mass transfer processes occurring prior to the adsorption intervention, i.e., diffusion across the liquid film surrounding individual particles and diffusion within the ion exchanger particle itself. The actual adsorption of OVA to DEAE Sepharose FF in fully mixed stirred vessels and in packed bed columns was consistent with both models. In the case of HSA, however, the adsorption profile in an agitated vessel was consistent only with the pore diffusion model and neither model could correctly predict the latter part of the breakthrough profile observed in packed bed experiments.