Abstract
The phase images of cells and tissues are of great importance for obtaining their morphological and dynamics information. However, because of the uniform optical path-length of a light field passing through the inhomogeneous cells whose nuclei’ shape and structure are different but with equivalent physical thickness, we can not identify their subgroup just from their phase images, since their wrapped phase images have similar shape. Thus we put out a new idea that uses their scattering distributions as a auxiliary criterion for identifying cells with different nuclei structure. We built the ellipsoidal models to denote the cells’ external shape and placed three kinds of nuclei, such as hexahedron, cylinder and spheroid, into the center of ellipsoids to compose nucleated leucocytes. Employing the VirtualLab software to carry out the simulations, we first prove that, for cells with different nuclei structures, their wrapped phase distributions indeed have similar shape. Then after analyzing the results of scattering simulations, we obtain some important scattering characteristics related with their different nuclei’ shape and structures which are very useful for helping us to identify blood cells’ subgroup and can also provide good guidance for experimental research on inhomogeneous cells.
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