Abstract
Production of extracellular laccase by the white-rot fungus Pycnoporus sanguineus was examined in batch submerged cultures in shake flasks, baffled shake flasks and a stirred tank bioreactor. The biomass growth in the various culture systems closely followed a logistic growth model. The production of laccase followed a Luedeking-Piret model. A modified Luedeking-Piret model incorporating logistic growth effectively described the consumption of glucose. Biomass productivity, enzyme productivity and substrate consumption were enhanced in baffled shake flasks relative to the cases for the conventional shake flasks. This was associated with improved oxygen transfer in the presence of the baffles. The best results were obtained in the stirred tank bioreactor. At 28°C, pH 4.5, an agitation speed of 600rpm and a dissolved oxygen concentration of ~25% of air saturation, the laccase productivity in the bioreactor exceeded 19UL(-1)days(-1), or 1.5-fold better than the best case for the baffled shake flask. The final concentration of the enzyme was about 325UL(-1).
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