Abstract
Aim. To study the morphofunctional state of ovaries after the introduction of recombinant FSH. Materials and methods. Two groups of mature female Wistar rats were used in the study. The experimental group consisted of 35 rats with a model of follicular ovarian cysts, while 25 rats were in the control group. Rats were taken out of the experiment on days 3, 7, 15, 30 and 60. Paraffin slides of ovaries were stained with hematoxylin and eosin by Van Gieson. Histological and morphometric investigations were performed. Results. On day 7 of FSH administration, the maximum increase in the size of the ovaries was observed due to the formation of single-cell follicular cysts. Rats in the experimental group showed a marked decrease in the number of growing follicles on day 7 and 15. The increase in atretic bodies and follicles in comparison with the control group was observed on day 7 and lasted until the end of the experiment. Additionally, on day 7 of the experiment, hyperemia and vasoconstriction were noted. The number of yellow bodies decreased during the experiment, and an increase in collagen formation occurred starting from day 15 of the experiment. Conclusion. The introduction of follicle-stimulating hormone for 7 days leads to disruption of folliculogenesis, strengthening of atresia in the ovaries, and the formation of functional cysts.
Highlights
Two groups of mature female Wistar rats were used in the study
The experimental group consisted of 35 rats with a model
while 25 rats were in the control group
Summary
Яичников в основной группе во все сроки эксперимента не отличалось от такового в контроле. На 7- и 15-е сут эксперимента отмечалось снижение количества растущих фолликулов по сравнению с таковым в группе контроля (р < 0,001). На 30- и 60-е сут отмечалось увеличение удельного объема атретических тел и фолликулов по сравнению с таковым в группе контроля (р < 0,001). Восстановление нормального фолликулогенеза не происходит в течение 60 сут после окончания эксперимента, наблюдается увеличение процессов атрезии и нарушение процессов рекрутирования растущих фолликулов, что закономерно снижает репродуктивный потенциал яичника. Мед наук, ассистент, кафедра акушерства и гинекологии, СибГМУ, г. Наук, профессор, кафедра гистологии, эмбриологии и цитологии, СибГМУ, г. For citation: Trus D.A., Tikhonovskaya O.A., Okorokov A.O., Mustafina L.R., Timofeeva O.S., Logvinov S.V., Gerasimov A.V., Varakuta E.Yu., Potapov A.V., Sukhanova G.A., Kutsenko I.G. Modeling of functional ovarian cysts by introducing a folliclestimulating hormone. Siberian State Medical University (SSMU) 2, Moskow Tract, Tomsk, 634050, Russian Federation
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