MODELING OF COMBINED EFFECTS OF THE EXPOSURE TO CHEMICALS (ALUMINUM) AND REGULATORY IMMUNE AND ENDOCRINE FACTORS IN RESEARCH ON CYTOKINES PRODUCTION IN EXPERIMENTS IN VITRO

Abstract

Introduction. Analysis of individual components of the network of regulatory neuroendocrine and immune interactions, as well as their possible combination with the use of mathematical modeling technology, makes it possible to identify the likely consequences of the negative impact of man-made environmental factors on public health and to determine optimal strategies for reducing morbidity. The aim of the work is to simulate cytokines production in vitro under the combined effect of chemical factors (aluminium) and regulatory immune and endocrine mediators. Material and methods. The experiment was performed on peripheral blood samples of healthy donors (n=68). An immune cell suspension was used, isolated by centrifugation in a ficoll-verografin density gradient. Viral load modeling was performed with a complex mitogen. The following factors were used: IL-1 β, cortisol, aluminum. The concentration of cytokines IL-8, IL-10, IL-17 was determined by enzyme immunoassay. Statistical analysis was performed using Statistica 6.0. Results. A significant change in the production of IL-8 and IL-10 was found in groups with different levels of experimental IL-1 exposure. The combined effect of IL-1 and high levels of cortisol and aluminum increased the levels of IL-8 and IL-10. In the study of the production of IL-17 inhibitory effects with increasing concentrations of IL-1 were revealed. The nonlinear equations of the concentrations dependences of the studied cytokines on the content of IL-1, cortisol and aluminum were determined with checking the models adequacy to the experimental data by the method of dispersion analysis. Discussion. The study of characteristics of the changes in immune cytokine mediators under the influence of physiological and chemical factors has revealed the interaction mechanism of a specific chemical environment and immune-endocrine regulation system components during the course of immunological processes in the body. Conclusion. The results of the study showed interrelations in the system of cytokine immune mediators associated with the increased production of IL-8 and IL-10 and a decrease in IL-17 under conditions of increasing concentrations of IL-1, as well as with the combined effect of cortisol and aluminum. Mathematical modeling has determined the nonlinear nature of the identified patterns.