Modeling of apoptosis through gene interaction network and analysis of gene expression pattern

Abstract

Apoptosis is one of the most life-essential processes to remove undesirable cells from the body and the first time coined by Kerr, Currie, and Wyllie in 1972. Apoptosis is primarily regulated by three major pathways such as Intrinsic, Extrinsic and Granzyme pathway. In this study design parameters of genes involved in apoptosis are retrieved for model development to check the stability of genes. Models of apoptosis pathways are developed to check the normal or abnormal function of genes. Then a network of apoptosis genes is generated to identify the most significant hub nodes by applying statistical measures. The simulation of each model shows the abnormal function of genes pointed out dysregulations except the granzyme pathway. In the result of a gene interaction network, 10 significant genes are identified based on degree distribution and modularity class. Those genes that are lying in 1 modularity class are included while those genes that are lying in 0 modularity class are excluded and their expression level is determined by modeling. This approach provides future research to understand any kind of perturbation involves in the different cellular pathways.