Abstract
The dynamic range of a polycratic reversed-phase liquid chromatographic (RPLC) method for measuring hydrophobicity constants (log k′ w) for non-ionic, organic molecules was examined. Evidence is presented that indicates that the differential hydrogen bonding effect present in most RPLC methods has been minimized by selection of appropriate stationary and mobile phase conditions. Thus, values of log k′ w determined for compounds of different net hydrogen bonding properties (non-congeners) can be compared directly. Results indicate that noncongeners congeners with hydrophobicities varying between −0.20 ≤ log k′ w ≤ 5.27 can be measured by this procedure. Additionally, the net hydrogen bonding property of any solute molecule can be predicted from the chromatographic experiment. Finally, the suppression of hydrogen-bonding discrimination effects in this method is shown to be a result of the low levels of octanol and alkylamine added to the mobile phase.
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