Abstract
Lipoprotein particles are aggregates of fat and protein whose purpose is to transport fats in the aqueous environment of plasma. Tracer kinetic studies and- the mathematical models developed to interpret the resulting data have provided many insights into the physiology and pathophysiology of lipoprotein metabolism. With the advent of better methodologies to quantitate stable isotopes, there has been a resurgence in metabolic studies. This has resulted in a need to restudy all aspects of tracer methodologies applied to lipoprotein metabolic studies. These will be discussed in the context of designing tracer kinetic metabolic studies with particular focus on (i) using radioactive and stable isotopic tracers, (ii) using endogenous and exogenous labeling techniques, and (iii) selecting modeling methodologies.
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