Modeling lipolysis in a reversed micellar system: Part I. Conventional batch reactor

Abstract

Triglyceride hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a batch-type reactor. The dynamic evolution of the product concentration was analyzed using several mechanistic models, both from the literature and developed in this work. A kinetic model with nonlinear product inhibition was found to be the most adequate for the description of batch hydrolysis data over an extensive range of time and substrate concentration. The obtained rate equation described the time course of not only the reactions performed in this work, at different water contents (W(0) = 7, 24) and pH values, but also the experimental results obtained in the literature with a Candida rugosa lipase.