Modeling and optimization of the effect of abiotic stressors on the productivity of the biomass, chlorophyll and lutein in microalgae Chlorella pyrenoidosa

Abstract

Microalgae have been exploited for food, biofuels, animal feed and pharmaceutical products over the last few decades. The microalgae biotechnology has grown and diversified significantly, despite these developments the number of commercially available products are still limited and thus there is a significant need to increase the production. Modeling and optimization are used to simulate the behavior of the studied problems and provide a basis for selecting optimum input settings that optimize the outputs. This paper investigates the effect of the beijerinck solution (BS), phosphate solution (PS) and hunter trace (HT) with different four levels (values) on the biomass productivity (BP), lutein productivity (LP) and total chlorophyll (TC) in microalgae Chlorella pyrenoidosa . A closer look at the effect of each level of the input factors on the output factors is given. Various modeling techniques, such as the spline model and Gaussian Kriging model, are investigated to recommend the optimal models for describing the relationships between the input factors and each output factor. The validation and efficiency of the recommended models are studied from various points of view. Based on the recommended models, the predicted values of the BP, LP and TC are given for all the possible level-combinations (conditions) of the BS, PS and HT and the best (optimal) level-combinations of the BS, PS and HT that maximize the BP, LP and TC are recommended. • This paper investigates the effect of BS, PS and HT on the BP, LP and TC in microalgae Chlorella pyrenoidosa. • A closer look at the significant effect of each level of these input factors on the output factors is given. • The potential relationships among these factors are investigated via various modeling techniques, such as linear, polynomial, spline and Kriging. • The predicted values of the BP, LP and TC are given for all the possible level-combinations (conditions) of the BS, PS and HT. • The best level-combinations of the BS, PS and HT which maximize the BP, LP and TC are obtained.