Abstract
The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study, the spouted bed dryer was utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the ratio of air flow rate to the minimum spouting air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at T = 50 °C and Q = 1.4. To investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that the optimum pH of DEE was decreased by 12.3% compared to FEE, while the optimum temperature of DEE compared to FEE increased by a factor of 85.7%. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.
Highlights
The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications
The results demonstrated that the inlet air temperature (p-value = 0.0), flow rate (p-value = 0.0), and their interaction (p-value = 0.005) were significant parameters in peroxidase drying process
The root ingredients of garden radish which is a rich source of peroxidase were extracted and the resulting paste was dried using the spouted bed dryer with inert glass beads
Summary
The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. To evaluate the effect of drying conditions on the REA, the experimental design was conducted at different inlet air temperatures and flow rates. To evaluate the activity of the dried peroxidase enzyme, the reaction of 0.6 mM TMB with 1 mM hydrogen peroxide in the presence of the DEE and FEE was carried o ut[31].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
