Model studies for isolation of G-quadruplex-forming DNA sequences through a pull-down strategy with macrocyclic polyoxazole

Keisuke Iida,Yue Ma,Tomomi Yokoyama,Yamato Tsushima,Kazuo Nagasawa,Kazunori Ikebukuro,Wataru Yoshida,Shadi Sedghi Masoud,Mai Sakuma

doi:10.1016/j.bmc.2019.02.056

Model studies for isolation of G-quadruplex-forming DNA sequences through a pull-down strategy with macrocyclic polyoxazole

Keisuke Iida, Yue Ma + Show 7 more

https://doi.org/10.1016/j.bmc.2019.02.056

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Journal: Bioorganic & medicinal chemistry	Publication Date: Mar 1, 2019
Citations: 5

Affiliation: Chiba University, Tokyo University of Agriculture and Technology, Tokyo University of Technology

#Pull-down Strategy #G4 Structures + Show 8 more

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Abstract

G-quadruplexes (G4s) are non-B DNA structures present in guanine-rich regions of gene regulatory areas, promoters and CpG islands, but their occurrence and functions remain incompletely understood. Thus, methodology to identify G4 sequences is needed. Here, we describe the synthesis of a novel cyclic hepta-oxazole compound, L1Bio-7OTD (1), bearing a biotin affinity-tag as a tool to pull down G4 structures from mixtures of G4-forming and non G4-forming DNA sequences. We confirmed that it could pull down G4s associated with telomeres, bcl-2 gene, and c-kit gene.

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