Abstract
In this note, we present a discussion of the advantages and scope of model-free analysis methods applied to the popular solvatochromic probe LAURDAN, which is widely used as an environmental probe to study dynamics and structure in membranes. In particular, we compare and contrast the generalized polarization approach with the spectral phasor approach. To illustrate our points we utilize several model membrane systems containing pure lipid phases and, in some cases, cholesterol or surfactants. We demonstrate that the spectral phasor method offers definitive advantages in the case of complex systems.
Highlights
Experimental results, such as fluorescence data, are examined and explained on the basis of different models
We present a discussion of the advantages and scope of model-free analysis methods applied to the popular solvatochromic probe LAURDAN, which is widely used as an environmental probe to study dynamics and structure in membranes [1, 2]
To emphasize the novelty of the spectral phasor approaches and to demonstrate the basic concept behind the methods, in this letter we offer a description of the strength of the method to study complex fluorescence emission in a simple and straightforward manner
Summary
Experimental results, such as fluorescence data, are examined and explained on the basis of different models. Sometimes an appropriate model is difficult to construct. We present a discussion of the advantages and scope of model-free analysis methods applied to the popular solvatochromic probe LAURDAN, which is widely used as an environmental probe to study dynamics and structure in membranes [1, 2]. To understand the use of LAURDAN as a sensor in membranes we need to understand the photophysical processes involved. The generalized polarization (GP) function [3] most commonly used with LAURDAN is based on the classical mathematics of fluorescence polarization. GP is defined as: GP = IIBB −+ IIRR , (1)
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