Abstract
Optimum conditions to maximize the observed resolution and signal-to-noise ratio of a scanning densitometer for high-performance thin-layer chromatography are described. Transmission measurements are compared in three operating modes; two being pseudo dual-beam methods and the third a dual-wavelength single-beam mode. In all modes resolution is shown to be independent of those parameters which control the dimensions of the measuring beam within their accessible ranges. Signal-to-noise ratios depend on both the operating mode and the dimensions of the measuring beam. In particular, signal shows a marked dependence on the ratio of slit height to spot diameter. Similar values for resolution and better signal-to-noise characteristics may be obtained in the transmission mode compared to reflectance for substances absorbing in the visible range.
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