Mode of interaction of two fluorinated-hydrogenated hybrid amphiphiles with dipalmitoylphosphatidylcholine (DPPC) at the air–water interface

Abstract

Two-component Langmuir monolayers formed on 0.02 M Tris buffer solution (pH 7.4) with 0.13 M NaCl at 298.2 K were investigated for two different fluorinated-hydrogenated hybrid amphiphiles (F6PH5PPhNa and F8PH5PPhNa or F6 and F8, respectively) with DPPC. Surface pressure ( π), surface potential (Δ V) and dipole moment ( μ ⊥) as a function of molecular surface area ( A) were measured by employing the Whilhelmy method and an ionizing electrode method. From the A– and Δ V– X F6 (or X F8) curves, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined as a function of surface mole fraction ( X F n ) at discrete surface pressures. Then, the behavior of occupied surface areas and surface potentials of the respective components could be made clearer. Compressibility ( C s), elasticity ( C s − 1 ) , and excess Gibbs energy (Δ G (ex)) as a function of X F6 (or X F8) were estimated at definite pressures. These physico-chemical parameters were found to reflect the mechanical strength of monolayer films formed. The regular solution theory being applied to Δ G (ex), the activity coefficients ( f) as well as the interaction parameter ( I p) between DPPC and two hybrid amphiphiles in the binary monolayers were evaluated. I p values thus obtained indicated that F8 molecules interact more strongly with DPPC molecules than F6. Moreover, in order to better understand the morphological monolayer state, Langmuir–Blodgett (LB) films made from DPPC and fluorinated-hydrogenated hybrid amphiphiles were examined by atomic force microscopy (AFM). The miscibility of the two components in the monolayer state is evidenced by these thermodynamic quantities and AFM observations. Furthermore, AFM images demonstrated that F8 could more effectively disperse the ordered domains of DPPC than F6.