Abstract
The degradation products of hyaluronic acid and sodium chondroitin sulphate by testicular and bacterial spreading factors were analyzed simultaneously by paper chromatography and reductimetry; then by spectrophotometry. The products obtained were found to be different qualitatively and quantitatively, thus confirming a different mode of action. The bacterial enzyme produced unsaturated disaccharides with hyaluronic acid while more polymerized compounds were obtained with testicular enzyme on both substrates.
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