Abstract
Different methods were used to elucidate the mode of action of thuricin S, a new class IId bacteriocin produced by Bacillus thuringiensis subsp. entomocidus HD198. According to cell viability tests, thuricin S was shown to exert a bactericidal effect on the sensitive cells of Bacillus thuringiensis subsp. darmastadiensis 10T. The use of the fluorescent probe 3,3'-dipropylthiadicarbocyanine iodide as an indicator proved that thuricin S interacts with the cytoplasmic membrane to dissipate the transmembrane potential. It was also demonstrated that thuricin S acts as a pore-forming bacteriocin, since it allows the nonpermeable stain propidium iodide to enter the cells. The loss of membrane integrity and the morphological changes in sensitive cells were visualized by scanning electron microscopy.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
