Abstract
Darkness and GA3 stimulate the elongation of the first internode but inhibit the production of roots while IAA inhibits internodal elongation but promotes the production of roots on epiphyllous buds of Bryophyllum tubiflorum. Cycloheximide inhibits both, implicating the synthesis of proteins in the growth of both root and shoot. Even pre-treatment of buds with cycloheximide for 4 h inhibits rooting as well as internodal elongation when these are subsequently transferred to IAA or GA3. On the other hand, pre-treatment with IAA or GA3 even for 8 h does not alleviate the inhibitory effect of cycloheximide suggesting that there is a lag period between the application of these regulators and the synthesis of proteins caused by them.
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