Abstract
In the hydrolysis of pullulan by a rice debranching enzyme, oligosaccharides intermediately produced were more rapidly degraded than expected from the random cleavage (relative maximum velocities: hexaose 3.0, nonaose 1.7, oligosaccharides of higher polymerizations 1.2, pullulan 1.0), and the final product maltotriose accumulated extraordinarily even at early stages of the hydrolysis. Therefore, the hydrolytic action on pullulan was confirmed to proceed in an endo-fashion with the incomplete random cleavage.
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