Mode of action for a new potential fungicide candidate, 3-(4-Methyl-1,2,3-thiadiazolyl)-6-trichloromethyl-[1,2,4]-triazolo-[3,4-b][1,3,4]-thiadiazole by iTRAQ

Abstract

ABSTRACTThe mode of action is the basis for the rational design of the novel agrochemicals. 3-(4-Methyl-1,2,3-thiadiazolyl)-6-trichloromethyl [1,2,4]triazolo [3,4-b][1,3,4]thiadiazole (YZK-C22) is a novel fungicide candidate. The aim of this study was identification of the mechanism of YZK-C22 against fungi. The results indicated that YZK-C22 inhibited the mycelial growth and spore germination of Botrytis cinerea. Total 56 differentially expressed proteins (DEPs) including 27 up regulated and 29 down regulated proteins were identified by isobaric tags for relative and absolute quantification (iTRAQ). The DEPs mainly concentrated on metabolic pathways, and the results were consistent across the studies of RNA and protein platforms. The pyruvate kinase (PK) activity in the glycolytic pathway was inhibited over 50% by 20 μg/mL of YZK-C22. Molecular docking analysis also validated that YZK-C22 could dock into the active centre of the PK instead of phosphoenolpyruvate. In summary, iTRAQ was an effective way for target identification of YZK-C22.