Mobilization Strategies for Autologous Collection and Cryopreservation of Peripheral Blood Stem Cells in Patients with Lymphoproliferative Diseases

Abstract

Background. Peripheral blood stem cells (PBSC) have largely replaced conventional, unprimed bone marrow (BM) as source for autologous transplantation. Aim. To present the mobilizing strategies in patients with lymphoproliferative diseases taking in consideration demographic and treatment related variables that have influence as prognostic factors. Material and Methods. The study was accomplished with patients treated with autologous transplantation at University Hematology Hospital, Skopje, and Republic of Macedonia during 5 year period. A total of 70 patients with lymphoproliferative diseases were included in this study. Results. Patients transplanted with PBSC, compared with patients transplanted with BM showed p<0.0001 for febrile days, number of microbiological solates, days of hospital stay, engraftment. Analysis of the parameters concerning the mobilization of PBSC in the analyzed group of patients revealed p<0.001 for WBC count before aphaeresis, number of procedures, day of aphaeresis in the G-CSF mobilized group and higher lymphononocyte count in the group mobilized with CT plus G-CSF. Conclusion. There are a number of factors that have been associated with the likelihood of succeseful stem cell mobilization. Such prognostic factors can assist clinicians in designing treatment regimens leading up to the transplant procedure and also identifying patients at high risk for failing the mobilization regimen.