Abstract
One of the most efficient and versatile mutagenesis methods used in Drosophila melanogaster (the fruit fly) relies on the mobilization of artificial derivates of P transposon. Aiming to reveal the biological significance of the patterns of transposition specific to P mobile element, mobilization of P{EP} artificial derivative was induced with a ?2-3 source in both male and female germline of D. melanogaster. By transposition of the viable and fertile insertion P{EP}3313, located in 68B1 at 592 pb downstream from 3’UTR of CG6199 gene, 46 new transgenic strains were constructed, each of them containing diverse reinsetions of P{EP}. Here we describe a combination of genetic and molecular experiments aiming to detect if conservation of the original insertion P{EP}EP3313 is associated with reinsertions in these mutant lines. A triplex PCR screening identified 22 mutant lines with conservation of P{EP}EP3313, a figure standing for 47,83 % of the transposition phenomena taken into account.
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Schedule a callMore From: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies
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