Mobile charges in the cell membranes ofHalicystis parvula

Abstract

Charge-pulse experiments were performed on cells of the giant marine algaHalicystis parvula. At normal pH (8.2), the voltage decay following a charge-pulse of 500 ns duration fed to the vacuole could be described by summing two exponential relaxations. The amplitudes and time constants of these relaxations were widely separated. The parameters of the two relaxation processes were found to be pH-dependent. Reduction of the external pH value from pH 8.2 to 5 resulted in a complete change of the two relaxation processes within a few minutes. Only one relaxation process could be observed at pH 5, within the time resolution of our instrumentation. The experimental data could not be explained by a two-membrane model with reasonable values for the specific capacitances of tonoplast and plasmalemma. The results of the charge-pulse relaxations were found to be consistent with the assumption that both membranes have very similar electrical properties and that both contain mobile charges with a total surface concentration of about 30 nmol·m(-2) and a translocation-rate constant of about 500·s(-1). The mobile charges became neutralized at pH 5 hhich led to a decrease of the apparent specific capacitance of the algal cells. They are presumably either part of a transport system for cations or connected with the chloride pump ofHalicystis parvula.