MO379: Collagen-Derived Peptides in CKD: A Link to Fibrosis

Abstract

Abstract BACKGROUND AND AIMS Collagen is a major component of the extracellular matrix (ECM) and has an essential role in the onset and progression of fibrosis and chronic kidney disease (CKD). Collagen alpha-1(I) (col1a1) is the most abundant collagen type in humans and the involvement of col1a1in CKD onset and progression is well established. We aimed to assess in detail the association of urinary col1a1 fragments with the progression of CKD and ageing. METHOD About 5000 urinary peptidomic datasets from healthy participants and CKD patients were retrieved from the ‘Human Urinary Proteome Database’. Inclusion criteria were age >18 years and eGFR being available. Urinary col1a1 fragments were identified and their abundance and correlation with eGFR and age were investigated. RESULTS Of 707 specific col1a1 peptides, 63 were significantly and highly positively associated with eGFR, while only six peptides showed a significant and strong negative association. A similar tendency was observed for ageing, where the abundance of most of the 244 peptides significantly associated with age, decreased with increasing age. The ten most significantly correlated peptides associated with eGFR corrected for age or age in a cohort matched for eGFR are listed in the table. CONCLUSION The results show a strong association between the reduced abundance of urinary collagen peptides and loss of kidney function as well as ageing. The data suggest that the col1a1 peptides indicate reduced degradation of collagen in CKD and aging. The results further suggest that fibrosis, potentially also of other organs, may be the consequence of attenuation of collagen degradation, and not increased synthesis.