Abstract
M2a was four fold increased. Interleukin-6 transcript level in inflammatory M1 cells was reduced two fold after polarization with IFN-γ and TSA. In M2a cells expressed significantly lower HDAC 7 levels compared to M1 cells (p<0.05) after 3 days of polarization. Human M1, M2a and M2c macrophages expressed the polarization markers CD64, CD200R and CD163 respectively. No significant differences were found in HDAC 1-11 and sirtuin 1 expression levels between MDM subsets directly after polarization. LPS stimulation of MDM in the presence of TSA showed that HDAC inhibition had no effect on TNF-α or IL-6 secretion, but IL-10 secretion was abrogated almost completely in all polarized macrophage MDM subsets. CONCLUSIONHDAC activity is involved inmacrophage polarization process. Inhibition of HDAC activity changes cytokine responses of macrophage M2a to inflammatory stimuli. Together, these data underscore the potential of HDAC inhibition to intervene in macrophage differentiation and reactivity in inflammatory disease.
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