Mo2048 Regulation of Intestinal Permeability by MicroRNA-29a/B Cluster

Abstract

Brain-gut interaction plays a key role in pathophysiology of irritable bowel syndrome (IBS). We reported a genetic association between β3-aderenoceptor and cholinergic receptor muscarinic 3 polymorphisms in IBS (Hepato-Gastroenterol 2011;58:1474-1478). We examined the association of brain-gut peptides, such as calcitonin gene-related peptide (CGRP α, encoded by CALCA), transient receptor potential vanilloid-1 (TRPV-1) and transcription factor 7-like2 (TCF7L2) polymorphisms with IBS. Methods: DNA was obtained from 108 IBS patients; (53 diarrhea type IBS-D, 31 constipation type IBS-C, 8 mixed type IBS-M, 16 unsubtyped IBS-U) and 61 controls. For the analyses IBS-(M + U) was combined into one group (NonDNonC). CALCA, TRPV-1 and TCF7L2 polymorphisms were determined by the polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) method. Results: The frequencies of the three genotypes; CALCA, TRPV-1 and TCF7L2 were not significantly different between IBS patients and controls. The distribution of the three genotypes was not significantly different between IBS-D, IBS-C and NonDNonC. The three genotypes frequencies were not significantly different between males and females in IBS. The frequencies of the three genotypes were not significantly different between under and over 3 years of disease duration in IBS. The frequencies of the TRPV-1 genotypes in IBS were significantly different between over 65 years old and under 65 years old. The frequencies of the CALCA and TCF7L2 genotypes in IBS were not significantly different between patients over 65 years old and under 65 years old. Conclusions: TRPV-1 polymorphisms are likely associated with age in IBS. TRPV-1 polymorphisms could be associated with IBS. S-727 AGA Abstracts Mo2050 Colorectal Transit During Acute Episodes of Diverticulitis Antonella Toma, Luca Bertini, Emanuele Casciani, Gianfranco Gualdi, Enrico Corazziari Background. Acute diverticulitis of the sigmoid colon presents with pain and bowel alterations: being constipation or diarrhea reported by 50% and 25%-35% of the patients respectively (Kelley MT, 2008). The colorectal mechanism leading to these opposite bowel behaviors is likely associated with a different distribution of colorectal contents as consequence of the motor activity of the large bowel. Aim. To assess distribution of colorectal contents during an episode of acute diverticulitis. Methods. Patients having the first episode of diverticulitis and no diverticulitis complications (Hinchey 1a) were recruited. CT scans of the abdomen and pelvis were performed within 4 hours from the onset of the acute diverticulitis episode in 22 patients (F=9; mean age 55.4±16.8 yrs; range 23-85) admitted to the emergency department. CT-scan datasets were scanned with a 16 MDCT scanner and image analysis was conducted on the reconstructed thin-slice portal-venous phase. Controls were 32 patients (F=12; mean age 68.5±9.4 yrs; range 50-85) with asymptomatic diverticula of the sigmoid and submitted to CT scan evaluation for not acute extra-gastrointestinal disease conditions. The large bowel was subdivided into 7 segments: ascending colon, right transverse colon, left transverse colon, proximal descending colon, distal descending colon, sigmoid and rectum. The absence/presence of contents, liquid, gas or semiquantitative (+,++,+++) amount of solid feces, were assessed and reported independently by three observers. Interobserver agreement was substantial (Cohen's Kappa=0.73). Results. In comparison to controls, patients with acute diverticulitis showed more frequently (a) gas or absence of contents in the sigmoid colon (p≤0.05), (b) less solid feces (27% vs. 56%, p ≤0.05) and more gas (45% vs. 16%, p≤0.05) in the distal descending colon, (c) equal amount of solid feces and more liquid feces in the right part of the colon, from the ascending (36% vs. 9%, p ≤0.05) to midtransverse colon (23% vs. 0%, p≤0.01) and (d) more liquid feces in the rectum (27% vs. 3%, p ≤0.01). Summary. During episode of diverticulitis the distribution of large bowel contents indicates that (a) sigmoid colon often contains gas or no content, (b) feces, solid and liquid, as well as gas are retained proximal to the sigmoid, being the solid and liquid feces mainly retained in the right part of the colon ,and the gas mainly retained in the descending colon, and (c) liquid feces are mainly present in the rectum. Conclusive hypothesis. The inflamed sigmoid may cause (a) constipation by inhibiting right to left colon transit of solid feces and being of obstacle to transit of gas and liquid contents through the sigmoid and, (b) by inhibiting the absorption of water, there is an increased amount of liquid feces in the colorectal that leads to diarrhea.