Abstract
Autofluorescence (AF) is a means to distinguish between normal and diseased tissue, but its molecular basis is unclear and intensity-based contrast is often not sufficiently specific. Fluorescence lifetime imaging (FLIM) maps the decay rate of fluorescence emitted from tissue samples, providing quantitative AF contrast. FLIM has been recently implemented by our group in three endoscopic instruments consisting of a confocal laser endomicroscope, a wide-field fibre-optic endoscope and a single point fibre-optic probe.
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