Mo1233 Functional Evidence for the “Purse String Morphology” of the External Anal Sphincter

Abstract

tion, in C57Bl6 mice. In vivo water T2 was not different between the NASH mouse model (MCD) and controls (MCS), however, the T2 of liver lipids as well as lipid fractions were significantly elevated in the MCD mice. Ex vivo metabolite profiles could be separated using principle component analysis (PCA) from HR-MAS MRS tissue data. Peaks identified to contribute to the separation of the MCD and the MCS mice, were found to have significant correlations to peaks associated with fatty acids. Our results indicate that non-invasive MRS is a usefull tool in diagnosis NAS as liver-derived lipid transverse relaxation rate values could discriminate the MCD mice from the MCS mice in vivo. The ex vivo HR-MAS results suggested that this could be due to subtle differences in the lipid profiles between the MCD and MCS groups. In conclusion, we report non-invasive in vivo diagnostic approach to NASH in mice. Our results promise translational value for MRS imaging in investigating disparities in lipid profiles in NASH.