Abstract
Pleurotus eryngii and P. pulmonarius are being investigated for straw biopulping. They produce up to seven MnP isoenzymes which have been purified and characterized from solid-state fermentation (SSF) and liquid cultures. Pleurotus pulmonarius produces under both conditions a unique MnP, with similar pI but different molecular mass. However, P. eryngii secretes five MnP isoenzymes: three in SSF, differing in both molecular mass and pI, and two in liquid culture, differing in pI. The isoenzymes from each fungus differ in their N-terminal sequences. A noteworthy characteristic of six of them is their Mn-independent activity on 2,6-dimethoxyphenol and veratryl alcohol. Among them, those produced under liquid or SSF conditions share several characteristics, including two N-terminal consensus sequences. Finally, the isoenzyme with the highest pI, among those purified from P. eryngii SSF, is more related to. Phanerochaete chrysosporium MnP.
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