MNG promotes neurogenesis against cerebral ischemic injury (LB626)

Abstract

MNG is an active component of sarcocarp of C. officinalis that have shown neuroprotective effects. The aim of the present study is to test whether MNG promote neurogenesis via Wnt/β‐catenin signaling pathway for brain recovery in a rat model of focal cerebral ischemia. MNG were administered intragastrically once daily at the concentrations of 30, 90 and 270 mg/kg respectively for 7 days post‐ischemia. Ludmila Belayev score tests was performed to detect neurological function. Immunofluorescence staining of Nestin was used to identify the endogenous neural stem cells proliferation in the subventricular zone. The expression of proteins involved in and related to Wnt/β‐catenin signaling pathway was detected by western blotting analysis. MNG significantly promoted neurogenesis for brain recovery 7 days post ischemia. Increased expression of Wnt 3a and β‐catenin, along with activation of downstream transcription factors, indicated that the neurorestorative effects of MNG may be associated with Wnt/β‐catenin signaling pathway. These data provide support for understanding the mechanisms of MNG in neurorestorative effects and suggest a potential new strategy for stroke treatment.Grant Funding Source: Supported by the National Science and Technology Major Project 2012ZX09102201‐106.