Abstract
The Muller neuroglial cells in cultures of retina from newborn rabbits were investigated with special regard to their morphological and histochemical properties. The Muller cells were large in vitro and had a single nucleus with several nucleoli. Their processes showed webbings at the points of branching. Time-lapse cinematographic records proved that the cell body and the processes changed their shapes and positions with time in vitro. Electron microscopic studies on these cells demonstrated the presence of many glycogen granules, microtubules and some filaments. Processes of the Muller neuroglial cells usually formed a continuous membranous structure between the cells in the explant and the surrounding nutrient medium. The cells showed from a cytochemical point of view active glycolysis not only via the Embden-Meyerhof pathway but also via the hexose monophosphate shunt. The cultured Muller cells possessed high phosphorylase activity. The morphological and cytochemical characteristics of the Muller neuroglial cells are discussed in relation to those in the rabbit retina in vivo. It is concluded that the Muller cells develop and mature to a certain extent in vitro.
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