Mixture of tree species enhances stability of the soil bacterial community through phylogenetic diversity

Abstract

The composition of tree species might influence microbial diversity considerably, yet investigation of the consequences of changes in diversity for stability of the microbial community is still in its early stages. Understanding how diversity governs community stability is vital for predicting the response of an ecosystem to environmental changes. Phylogenetic diversity (PD) describes the distinct evolution of species in a community, and might be useful for estimating the effects of biodiversity on ecosystem function and stability. High‐throughput 16S rRNA gene sequencing was used to examine soil bacterial phylogenetic distances, phylogenetic diversity and interactions between individuals in five single‐species plantations and three mixed‐species plantations. The plantations were established on the same initial substrate, and sampling was at 68 relatively spatially independent sites. Our results showed that mixed tree species enhanced soil bacterial phylogenetic diversity and community stability, and that phylogenetic diversity had a positive effect on stability of the soil microbial community. We also found evidence that microbial communities characterized by distantly related species with weak interactions were more stable in mixed plantations than communities with strong interactions in single‐species plantations. These results may be explained by the “insurance hypothesis”, that large phylogenetic diversity of microbial communities, which share different ecological niches, insures them against decline in their stability. This is because, even if some microbial species fail to deal with environmental change, others might not necessarily be affected similarly. Our findings demonstrate that phylogenetic diversity is the main controlling factor of the variation in stability across sites and requires more attention in sustainable forest management.Highlights Mixed species considerably improved stability of the soil bacterial community. Bacterial phylogenetic diversity was greater in mixed‐ than in single‐species plantations. Mixed species resulted in weak microbial interactions in a community. There was a strong relation between phylogenetic diversity and stability.