Mixture of Honey, Beeswax and Olive Oil Inhibits Growth of Staphylococcus aureus and Candida albicans

Abstract

Honey, beeswax and olive oil mixture (1:1:1, v/v) is useful in the treatment of diaper dermatitis, psoriasis and eczema. The study was designed to investigate effects of honey, olive oil, and beeswax and the mixture on growth of Staphylococcus aureus and Candida albicans isolated from human specimens. The following experiments were performed: 1) honey mixture was poured on holes made on plates seeded with S. aureus or C. albicans, 2) the microorganisms were cultured onto media made of honey mixture alone, nutrient agar-honey mixture and Sabouraud glucose agar-honey mixture. The concentration of honey mixture in nutrient agar or Sabouraud glucose agar was 12.5, 25, 33, 50 and 66% (v/v), and 3) honey, olive oil or beeswax was added onto nutrient agar or Sabouraud glucose agar at a ratio of 1:2 (v/v) and then were seeded with S. aureus or C. albicans. Clear zone of inhibition was observed around holes filled with honey mixture; 3.5 mm on media seeded with C. albicans and 4 mm on media seeded with S. aureus. No growth of either microorganism was obtained on media made of honey mixture alone. The minimum concentration of honey mixture in nutrient agar-honey mixture media required to inhibit S. aureus was 50% and 66% concentration was required to inhibit C. albicans growth onto Sabouraud glucose agar-honey mixture media. No growth of S. aureus or C. albicans was obtained on media containing honey whereas mild to moderate growth was obtained on media containing olive oil or beeswax. Honey and honey mixture apparently could inhibit growth of S. aureus or C. albicans.