Abstract
The effects of glucose concentration and irradiance on mixotrophiccultivation of Platymonas subcordiformis were investigated in flaskcultures. The optimal glucose concentration was 24 g L-1, andirradiance was 95 μmol photon m-2 s-1. Based on theoptimization of culture conditions and a modified SK(IA) medium, themaximum biomass was 3.68 g L-1 after 14 days, which was about6 times that in autotrophic culture, and the specific growth rate reached0.62 d-1, twice that in autotrophic culture. Using the optimalconditions established in flasks, mixotrophic culture in a 5.0-Lphotobioreactor was achieved, and the yield after 8 days was 3.30 gL-1.
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