Mixed‐charge cellulose nanocrystal modified cellulose acetate membrane with endotoxin scavenging ability and antibacterial properties

Abstract

AbstractBlending modification is used to improve the hemocompatibility of cellulose acetate (CA) dialysis membranes while endowing the CA membrane with endotoxin scavenging and antibacterial capabilities. First, polydopamine (PDA) and polyethylenimine (PEI) are used as negatively charged and positively charged moieties, respectively, together with cellulose nanocrystals (CNCs) to construct mixed charge CNCs (PEI‐PDA@CNCs). Then PEI‐PDA@CNCs is blended with the CA casting solution, and the modified CA dialysis membrane is prepared through a phase inversion process. Through biocompatibility, adsorption, antibacterial, and filtration experiments, various properties of PEI‐PDA@CNCs modified CA membrane (PDCA) are systematically tested. The results show that the PDCA membrane exhibited good blood compatibility and noncytotoxicity. At the same time, the PDCA membrane has antibacterial properties and excellent endotoxin adsorption capacity. Compared with the CA membrane, the clearance rates of PDCA membrane for Staphylococcus aureus and Escherichia coli reach 54.0% ± 1.5% and 52.9% ± 5.9% respectively. In the dynamic adsorption experiment, the adsorption capacity of PDCA membrane for endotoxin increases to 2322.1 ± 45.9 EU/g. The research results indicate that PEI‐PDA@CNC‐modified multifunctional CA membrane with endotoxin adsorption and antibacterial properties has potential application prospects in the fields of hemodialysis and blood purification.